GENETIC PREDICTORS FOR THE FORMATION OF CHRONIC PURULENT OTITIS MEDIA

Abstract

84 patients with chronic suppurative otitis media (41 patients with mesotympanitis, 43 with epitympanitis) were examined. To verify the diagnosis, all subjects underwent standard general clinical, radiological, audiometric studies, molecular genetic analysis of DNA by PCR and immunological examination by ELISA. The obtained data were statistically processed using the STATISTICA 6.0 software package (StatSoftInc., USA). The critical level of significance when testing statistical hypotheses was taken to be less than 0.05. A statistically significant predominance of the homozygous C/C genotype and the heterozygous -T/C genotype for the high-producing allele C* of the IL-1β gene, respectively, in polymorphic loci 31 and 511 and homozygous genotypes A/A and C/C for low-producing alleles A* and C, respectively, was established. * of the IL-10 gene, respectively, in polymorphic loci -1082 and -819 in the group of patients with a carious-destructive course of chronic suppurative otitis media. High levels of IL-1β (p<0.05) were registered against the background of low levels of IL-10 (p<0.05) in blood serum in individuals with epitympanitis with high-producing variants of pro-inflammatory cytokine genes and low-producing alleles of anti-inflammatory cytokines. Lymphocyte-platelet depression was found in all patients with chronic suppurative otitis media.