INTRAORGAN CHANGES OF RAT SPLEEN VESSELS IN EARLY POSTNATAL ONTOGENESIS

Abstract

The aim is to study intraorgan changes in spleen vessels in white rats in early postnatal ontogenesis.
Materials and methods of research. The experiment was carried out on 90 male mongrel rats in the newborn and at 6, 11, 16 and 21 days of age. To study the histotopography of the processes, polyclonal antibodies to CD3 and CD20 (Ventana, Switzerland) were used at a dilution of 1:100, carried out on histological glasses with an adhesive coating (Ventana, Switzerland). In the preparations, the spleen structures were examined morphometrically using an ocular micrometer. The diameter of the periarterial lymphatic couplings of the spleen, lymph nodes and their germinal centers, the width of the mantle, marginal and periarterial zones of lymph nodes, the relative area of connective tissue elements and white pulp (relative to the total area of the cut) were measured.
Results. The spleen of newborn baby rats is functionally immature, lymph nodes are not fully formed, it is difficult to distinguish all zones in them and germinative centers are not determined. When performing IHC with CD 20 markers, it was found that the number of LF without breeding centers in the spleen of the control group of rats reaches a maximum by 6 days of age, and with breeding centers by 11 days of age.
Conclusions. It has been established that, starting from the age of nine months, involutive changes are observed, characterized by an increase in the number of connective tissue elements in the spleen, a decrease in germinal centers in lymph nodes, the total number of lymphocytes in lymph nodes without reproduction centers, periarterial lymphatic couplings.