FEATURES OF THE OCCURRENCE OF SINGLE-NUCLEOTIDE POLYMORPHISM IL10 (C592A) AMONG PATIENTS WITH PURULENT-INFLAMMATORY DISEASES OF THE MIDDLE EAR AND ASSESSMENT OF ITS CONTRIBUTION TO THE MECHANISMS OF DISEASE FORMATION
Keywords:purulent-inflammatory diseases of the middle ear, polymorphism of the IL10 gene (C592A),,, allele, frequency, genotype, share of carriage
The features of the single-nucleotide polymorphism of the IL10 (C592A) gene in purulent-inflammatory diseases of the middle ear were investigated. The polymorphism of the IL10 (C592A) gene was evaluated by analyzing DNA samples by setting up a polymerase chain reaction (PCR) in Real Time mode.
The results of the study on the characteristics of the polymorphism of the IL10 (C592A) gene showed that it was revealed that the minor A allele can contribute to the initiation of inflammatory processes that realize the onset of purulent-inflammatory diseases of the middle ear, increasing the risk of their development as a whole by 1.6 times (χ2 = 2.5; P = 0.2). Moreover, the association became apparent. Minor allele A (χ2 = 4.3; P = 0.05) with an increased risk of onset of mild course of purulent-inflammatory diseases of the middle ear. In addition, there was a tendency to increase the risk of formation of a mild course of the disease on the part of all three genotypes (C / C - χ2 = 3.2, P = 0.1; A / G - χ 2 = 1.3, P = 0.3; OR = 1.7; A / A - χ2 = 1.9, P = 0.2) of the polymorphic gene IL10 (C592A). Meanwhile, there are no significant associative relationships between the carriage of minor alleles A (χ2 = 0.5; P = 1.0), as well as genotypes C / A (χ2 = 0.2; P = 0.7) and A / A (χ2 = 0.2; P = 0.7) of the polymorphic il10 (C592A) gene and the development of purulent-inflammatory diseases of the middle ear of medium and severe course.