EXPERIMENTAL SUBSTANTIATION OF THE EFFECTIVENESS OF GLYCINE IN MYOCARDIAL INFARCTION

Abstract

The aim of the study was to evaluate the effect of the amino acid glycine on
the degree of damage to cardiomyocytes in myocardial infarction, which was assessed by
the activity of cardiospecific enzymes in the blood. The experiments were carried out on
male rabbits, in which experimental myocardial infarction was induced by ligation of the
descending branch of the left coronary artery. Immediately after ligation, an aqueous
solution of glycine (manufactured by MRPC Biotiki, RF) at a dose of 100 mg/kg of body
weight was orally injected into the stomach of experimental rabbits through a tube. Further,
the animals received glycine orally at the indicated dose every day. After 0.5, 1, 3, 6, 12,
24, 72 and 168 hours after coronary occlusion, the activity of MB-creatine phosphokinase
(MB-CPK), aspartate aminotransferase (AST) and lactate dehydrogenase (LDH) was
determined in blood plasma. The results showed that glycine at a dose of 100 mg/kg of body
weight led to a smaller increase in the activity of CF-CPK, LDH and AST in the blood
plasma of rabbits in the dynamics of experimental myocardial infarction. Also, in the blood
plasma of patients with myocardial infarction, the content of free amino acids was determined
by high performance liquid chromatography. It was found that in acute myocardial infarction,
the content of glycine in the blood is reduced by 39.7% compared with normal values.
Based on the results, a conclusion was made about the cardioprotective property of glycine
in necrotic myocardial damage.